CASE STUDIES View All
Stem Cell Differentiation
Customer Says:“The ability to generate hydrogels over a range of stiffnesses from a single material was a huge advantage for our metabolomics experiments as it allowed us to control stem cell growth and differentiation without the use of different media formulations,…
Improving Cartilage Phenotype from Differentiated Pericytes
Customer Says:“Having a biomaterial system that is able to influence phenotypic expression is hugely desirable as a replacement for chemically-induced differentiation methods, as being able to match the features of tissue produced in vitro to the required structure of the lost…
Culture and Maintenance of Primary Human Hepatocytes
Customer Says:“Biogelx manufactured a custom formulation hydrogel to our specification, which allowed us to find the optimal environment for our system to support hepatocyte function. Being able to achieve comparable results to Matrigel using a matrix that is consistent, with no…
PUBLICATIONS View All
The study highlights developments in hydrogel materials with biological responsiveness built in. These ‘smart’ biomaterials change properties in response to selective biological recognition events. When exposed to a biological target (nutrient, growth factor, receptor, antibody, enzyme, or whole cell), molecular recognition events trigger changes in molecular interactions that translate into macroscopic responses, such as swelling/collapse or solution-to-gel transitions. The hydrogel transitions may be used directly as optical readouts for biosensing, linked to the release of actives for drug delivery, or instigate biochemical signaling events that control or direct cellular behavior. Accordingly, bioresponsive hydrogels have gained significant interest for application in diagnostics, drug delivery, and tissue regeneration/wound healing.
Controlling cancer cell fate using localized biocatalytic self-assembly of an aromatic carbohydrate amphiphile
We report on a simple carbohydrate amphiphile able to self-assemble into nanofibers upon enzymatic dephosphorylation. The self-assembly can be triggered by alkaline phosphatase (ALP) in solution or in situ by the ALP produced by osteosarcoma cell line, SaOs2. In the latter case, assembly and localized gelation occurs mainly on the cell surface. The gelation of the pericellular environment induces a reduction of the SaOs2 metabolic activity at an initial stage (≤7 h) that results in cell death at longer exposure periods (≥24 h). We show that this effect depends on the phosphatase concentration and thus, it is cell-selective with prechondrocytes ATDC5 (that express ~15-20 times lower ALP activity compared to SaOs2) not being affected at concentrations ≤ 1 mM. These results demonstrate that simple carbohydrate derivatives can be used in an anti-osteosarcoma strategy with limited impact on the surrounding healthy cells/tissues.
Probing the Metabolomics of Stem Cell Differentiation with Biomaterials
In this issue of Chem, Ulijn, Dalby, and co-workers have developed a novel biomaterials platform as a screening tool for identifying metabolites that can bias differentiation of mesenchymal stem cells.
TECHNICAL DATA View All
Biogelx Powder – Preparation and Guidelines for Use in Bioprinting
The recommended preparation provided in this instruction leaflet will provide a printable Pre-Gel solution, capable of producing mechanically stable 3D structures.