competitive synthetic alternative to traditional animal-derived 3D cell culture products
Biogelx™ hydrogel technology is based on simple, short self-assembling peptides. Typical products combine a structural, fiber-forming peptide with functional peptides, which introduce (bio-) chemical functionality to the surface of the fibers. The result is a nanofibrous network with a similar nanoscale matrix structure to that of native extracellular matrix (ECM) in human tissue. The beauty of this simple peptide technology is that both the mechanical and chemical properties of the hydrogels can be independently adjusted to provide an optimal environment for the culture of a variety of cell types. In addition to being able to tune the stiffness of the hydrogels to match a broad range of tissue types, products which incorporate biomimetic peptide sequences of key ECM proteins such as fibronectin, laminin, and collagen are available to encourage cell-matrix interactions. The ability to incorporate such functionality allows Biogelx™ hydrogels to provide synthetic yet biologically-relevant alternatives to animal-derived 3D matrices such as Matrigel and collagen.
CORE TECHNOLOGY | PRODUCT BENEFITS
Biogelx™ technology is based on a two-peptide system: a hydrophobic ‘gelator’ peptide (Fmoc-diphenylalanine), and a hydrophilic ‘surfactant’ (Fmoc-serine). They self-assemble to form fibers in aqueous environments. In the presence of Ca2+ions these peptide nanofibers crosslink to form hydrogels that are >95% water and possess a similar structure to natural ECM. The peptide fibers which make up our gels present hydrophilic functionality on the surface, thereby presenting appropriate surface chemistry for cell adhesion. The “surfactant” functionality of the peptide fibers can be modified to incorporate various biomimetic peptide sequences from key extracellular matrix proteins such as fibronectin (RGD), laminin (IKVAV, YIGSR), and collagen (GFOGER). Such peptide motifs have been proven to significantly enhance cell attachment and subsequent cell growth.